小鼠血清白蛋白，albumin/小鼠血清白蛋白

Description  Chromatographically purified mouse albumin

Product code  MAlb

Biological origin  Mouse serum

Physical form  Lyophilized, stable white powder.

Identity  Albumin is a stable small polypeptide with a strong antigenicity. It has 
a  molecular  weight  of  69,000  and  an  isoelectric  point  of  5.7,The protein is isolated  from serum by sequential precipitation and further purified  by  ion  exchange  chromatography  and  affinity chromatography.  The  identity  is  confirmed  and  tested  by  immuno-electrophoresis  and  radial  immunodiffusion  using  antisera  to  total serum proteins, immunoglobulins  and albumin.

Purity  Not less than 98% as determined by agar-gel electrophoresis. Tested 
in a concentration of 50 mg/ml at the level of sensitivity of the applied immunoprecipitating  techniques,  this  product  contains  traces  of  two alpha lines.

Preservative  No preservative added.

Intended use  Purified  mouse  albumin  may  be  used  as  a  reference  antigen, 
calibrator,  coating  protein  and  blocking  agent  in  a  variety  of immunoassays  including  immunodiffusion,  immunoelectrophoresis, ELISA,  Western  blotting,  dot-immunobinding  assays  (DIBA), haemagglutination, and cell-binding assays. 

Handling  This  product  is  shipped  lyophilized  at  ambient  temperature.  After 
arrival  store  at  +4°C.  Prolonged  storage  may  be  at  -20°C.  After opening  the vial,  the  lyophilized content  is  reconstituted by adding 1 ml of sterile distilled water, mixed gently by  inversion until complete dissolution  is obtained. Allow  to stand at ambient  temperature  for 5-10  minutes  to  reach  equilibrium.  Reconstituted  solution  may  be stored  frozen.  Working  dilutions  may  be  prepared  by  adding  the required amount of phosphate buffered saline  (PBS), should not be refrozen and preferably used within 24 hours. Repeated thawing and freezing  should  be  avoided.  If  a  slight  precipitation  occurs  upon storage,  this should be  removed by centrifugation and will not affect the performance of the product.

Packing  Vial with 10 mg mouse albumin lyophilized from 1 ml PBS (pH 7.2).

Storage / shelf life 

Lyophilized at +4° C                               at least 10 years 

reconstituted at or below -20° C            3-5 years 

reconstituted at +4° C                            7 days 
                             
Caution  This  product  should  be  handled  only  by  qualified  persons  and 
appropriate precautions should be taken in its handling and disposal, and  of  all  associated  materials.  For  in  vitro  laboratory  research purposes only.